Agarose Gel Electrophoresis of Low Molecular Weight DNA

This procedure performed in conjuction with DNA content provides reliable and reproducible results in the analysis of programmed cell death. In addition to verifying the results obtained by flow cytometry this method does not require the sorting of apoptotic regions to obtain low molecular weight DNA which is present in cells undergoing DNA fragmentation. Cells are fixed in 70% ethanol for DNA Content and an aliquot is reserved for the below procedure beginning with step 3.

Low Molecular Weight DNA Protocol

Reagents

References

Gong, J., Traganos, F., and Darzynkiewicz, Z. (1994) A Selective Procedure for DNA Extraction from Apoptotic Cells Applicable for Gel Electrophoresis and Flow Cytometry Analyt. Biochem., 218: p 314-319

Robinson, J. Paul, Mng. Ed.: Current Protocols in Cytometry 1998, John Wiley & Sons, Inc. New York, NY p. 7.5.10

DNA Content and Apoptosis 'TUNEL' Assay with anti-BrdU Annexin-FITC/PI

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Last updated: 11-March-04

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