Example of Dual Fluorescence from Research Core 3: Molecular Genetics and Environmental Carcinogenesis Cheryl Walker, Ph.D., Elena Kleymenova, Ph.D., Hiroyuki Kugo, Ph.D., & Kaoru Kiguchi, M.D., Ph.D.

In Tsc2 knockout rats that develop severe polycystic kidney disease, lack of tuberin resulted in loss of polycystin-1 localization to the lateral cell membrane and sequestration of polycystin-1 to the Golgi. The cover shows restoration of cell membrane-localized polycystin-1 (green) after tuberin-negative cells from Tsc2-/- polycystic kidneys were treated in vitro with brefeldin A. Brefeldin A disrupted the Golgi, as shown by Golgi marker p58 (red), and fused TGN with early endosomes, permitting polycystin-1 to exit the Golgi and traffic to the lateral cell membrane.

Kleymenova, E., Ibraghimov-Beskrovnaya, O., Kugo, H., Everitt, J., Xu, H., Kiguchi, K., Landes, G., Harris, P., and Walker, C. (2001) Tuberin-Dependent Membrane Localization of Polycystin-1: A Functional Link between Polycystic Kidney Disease and the TSC2 Tumor Suppressor Gene Molecular Cell 7:4 p. 823-832

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