UT, MD Anderson, Science Park - Research Division Flow Cytometry References

References*

1. Darzynkiewicz, Z., & Crissman, H.A., Ed.s: Flow Cytometry, Vol. 33 Methods in Cell Biology 1990, Academic Press, Inc., San Diego, CA

2. Givan,Alice L.: Flow Cytometry First Principles 1992, Wiley-Liss, Inc. New York, NY

3. Melamed, M.R., Lindmo, T., & Mendelsohn, M.L., Ed.s: Flow Cytometry and Sorting, Second Ed. 1990, Wiley-Liss, Inc., New York, NY

4. Ormerod, M.G., Ed.: Flow Cytometry A Practical Approach, Second Ed. 1992, Oxford University Press, Inc., New York, NY

5. Robinson, J. Paul, Ed.: Handbook of Flow Cytometry Methods 1993, Wiley-Liss, Inc. New York, NY

6. Shapiro, Howard M.: Practical Flow Cytometry, Third Edition 1995, Wiley-Liss, Inc., New York, NY

7. Linscott's Directory of Immunological and Biological Reagents, 11th Edition

8. Robinson, J. Paul, Mng. Ed.: Current Protocols in Cytometry 1998, John Wiley & Sons, Inc. New York, NY

Free On-Line Access

Shapiro, Howard M.: Practical Flow Cytometry, Fourth Edition 2005, Wiley-Liss, Inc., New York, NY

* References 1-8 are available as part of our service and located in the core facility.

Apoptosis

Kerr, J.F.R., Wyllie, A.H., and Currie, A.R. (1972) Apoptosis: A Basic Biological Phenomenon With Wide Ranging Implications In Tissue Kinetics Br. J. Cancer 26: p.239-257

Del Bino, G., Lassota, P., and Darzynkiewicz, Z. (1991) The S-Phase Cytotoxicity of Camptothecin Exp. Cell Res. 193: p.27-35

Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lassota, P. and Traganos, F. (1992) Features of Apoptotic Cells Measured by Flow Cytometry Cytometry 13:8 p.795-808

Telford, W.G., King, L.E., and Fraker, P.J. (1994) Rapid Quantitation of Apoptosis In Pure Heterogeneous Cell Populations Using Flow Cytometry J. Immunol. Meth. 172: p.1-16

Gong, J., Traganos, F., and Darzynkiewicz, Z. (1994) A Selective Procedure for DNA Extraction from Apoptotic Cells Applicable for Gel Electrophoresis and Flow Cytometry Analyt. Biochem. 218: p.314-319

Li, X., Melamed, M.R., and Darzynkiewicz, Z. (1996) Detection of Apoptosis and DNA Replication by Differential Labeling of DNA Strand Breaks with Fluorochromes of Different Color Exp. Cell Res. 222:1 p.28-37

Darzynkiewicz, Z., Juan, G., Li, X., Gorczyca W., Murakami, T., and Traganos, F. (1997) Cytometry in Cell Necrobiology: Analysis of Apoptosis and Accidental Cell Death Cytometry 27:1 p.1-20

O'Brien, M.C., Healy Jr., S.F., Raney, S.R., Hurst, J.M., Avner, B., Hanly, A., Mies, C., Freeman, J.W., Snow, C., Koester, S.K., and Bolton, W.E. (1997) Discrimination of Late Apoptotic/Necrotic Cells (Type III) by Flow Cytometry in Solid Tumors Cytometry 28:1 p. 81-89

Koester, S.K., Schlossman, S.F., Zhang, C., Decker, S.J., and Bolton, W.E. (1998) APO2.7 Defines a Shared Apoptotic-Necrotic pathway in a Breast Tumor Hypoxia Model Cytometry 33:3 p.324-33

McCloskey, T.W., Chavan, S., Tamma, S.M.L., and Pahwa, S. (1998) Comparison of Seven Quantitative Assays to Assess Lymphocyte Cell Death During HIV Infection: Measurement of Induced Apoptosis in Anti-Fas-Treated Jurkat Cells and Spontaneous Apoptosis in Pheripheral Blood Mononuclear Cells from Children Infected with HIV AIDS Res. and Hu. Retro. 14:16 p.1413-1422

Van Engeland, M., Nieland, L.J.W., Ramaekers, F.C.S., Schutte, B., and Reutelingsperger, C.P.M. (1998) Annexin V-Affinity Assay: A Review on an Apoptosis Detection System Based on Phosphatidylserine Exposure Cytometry 31:1 p.1-9

King, M.A., Radicchi-Mastroianni, M.A., and Wells, J.V. (2000) There is Substantial Nuclear and Cellular Disintegration Before Detectable Phosphatidylserine Exposure During the Camptothecin-Induced Apoptosis of HL-60 Cells Cytometry 40:1 p.10-18

Clarke, R.G., Lund, E.K., Johnson, I.T., and Pinder, A.C. (2000) Apoptosis Can Be Detected in Attached Colonic Adenocarcinoma HT29 Cells Using Annexin V Binding, But Not by TUNEL or Sub-G0 DNA Content Cytometry 39:2 p. 141-150

Darzynkiewicz, Z., Robinson, J., & Crissman, H. Ed.s Methods in Cell Biology Vol. 63, 3^rd Edition; 2001, Academic Press, Inc., San Diego, CA, Darzynkiewicz, Z., Bedner, E., and Traganos, F. Difficulties and Pitfalls in Analysis of Apoptosis: p. 527-546

Autophagy

Traganos, F., and Darzynkiewicz, Z. (1994) Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations. Methods in Cell Biology Vol. 41 p.185-194

Kanzawa, t., Kondo, y., Ito, H., Kondo, S., and Germano, I. (2003) Induction of autophagic cell death in malignant glioma cells by arsenic trioxide. Cancer Res. 63:9 p.2103-2108

Takeuchi, H., Kondo, Y., Fujiwara, K., Kanzawa, T., Aoki, H., Mills, G.B., and Kondo, S. (2005) Synergistic augmentation of rapamycin-induced autophagy in malignant glioma cells by phosphatidylinositol 3-kinase/protein kinase B inhibitors. Cancer Res. 65:8 p.3336- 3346

Bcl-2

Gratiot-Deans, J., Merino, R., Nunez, G., and Turka, L.A. (1994) Bcl-2 Expression During T-Cell Development: Early Loss and Late Return Occur at Specific Stages of Commitment to Differentiation and Survival Proc. Natl. Acad. Sci. USA 91: p.10685-10689

Dobmeyer, T.S., Klein, S.A., Dobmeyer, J.M., Raffel, B., Findhammer, S., Hoelzer, D., Helm, E.B., Rossol, R., and Kabelitz, D., (1998) Differential Expression of bcl-2 and Susceptability to Programmed Cell Death in Lymphocytes of HIV-1 Infected Individuals Clin. Immunol. & Immunopath. 87:3 p.230-239

BrdU for Absolute S-Phase

Gratzner, H. (1982) Monoclonal Antibody against 5-bromo- and 5-iodo-deoxyuridine: A New Reagent for Detection of DNA Replication Science 218: p.474-475

Dolbeare, F., Gratzner, H., Pallavicini, M.G., and Gray, J.W. (1983) Flow Cytometric Measurement of Total DNA Content and Incorporated BromodeoxyUridine Proc. Natl. Acad. Sci. USA 80: p.5573-5577

Terry, N.H.A., White, R.A., Meistrich, M.L., and Calkins, D.P. (1991) Evaluation of Flow Cytometric Methods for Determining Population Potential Doubling Times Using Cultured Cells Cytometry 12: p.234-241

Carlton, J.C., Terry, N.H.A., and White, R.A. (1991) Measuring Potential Doubling Times of Murine Tumors Using Flow Cytometry Cytometry 12: p.645-650

Li, X., Traganos, F., Melamed, M.R., and Darzynkiewicz, Z. (1994) Detection of 5-bromo-2-deoxyuridine Incorporated into DNA by Labeling Strand Breaks Induced by Photolysis (SBIP) Int. J. Oncology 4: p.1157-1161

Cyclins

Gong, J., Li, X., Traganos, F., and Darzynkiewicz, Z. (1994) Expression of G₁ and G2 Cyclins Measured in Individual Cells by Multiparamater Flow Cytometry: A New Tool in the Analysis of the Cell Cycle Cell Prolif. 27: p.357-371

Gong, J., Bhatia, U., Traganos, F., and Darzynkiewicz, Z. (1995) Expression of Cyclins A, D2 and D3 in Individual Normal Mitogen Stimulated Lymphocytes and in MOLT-4 Leukemic Cells Analyzed by Multiparameter Flow Cytometry Leukemia 9: p.893-899

Sramkoski, R.M., Wormsley, S.W., Bolton, W.E., Crumpler, D.C., and Jacobberger, J.W. (1999) Simultaneous Detection of Cyclin B1, p105, and DNA Content Provides Complete Cell Cycle Phase Fraction Analysis of Cells that Endoreduplicate Cytometry 35:3 p.274-283

DNA Content and Cell Cycle

Darzynkiewicz, Z., and Traganos, F. (1990) Multiparameter Flow Cytometry in Studies of the Cell Cycle from Melamed, M.R., Lindmo, T., & Mendelsohn, M.L., Ed.s: Flow Cytometry and Sorting, Second Ed. 1990, Wiley-Liss, Inc., New York, NY

Schmid, I., Uittenbogaart, C.H., and Giorgi, J.V. (1991) A Gentle Fixation and Permeabilization Method for Combined Cell Surface and Intracellular Staining with Improved Precision in DNA Quantification Cytometry 12: p.279-285

Toba, K., Winton, E.F., and Bray, R.A. (1992) Improved Staining Method for the Simultaneous Flow Cytofluorometric Analysis of DNA Content, S-Phase Fraction, and Surface Phenotype Using Single Laser Instrumentation Cytometry 13:1 p.60-67

Shankey, T.V., Rabinovitch, P.S., Bagwell, B., Bauer, K.D., Duque, R.E., Hedley, D.W., Mayall, B.H., and Wheeless, L. (1993) Guidelines for Implementation of Clinical DNA Cytometry Cytometry 14: p.472-477

Li, X., Melamed, M.R., and Darzynkiewicz, Z. (1996) Detection of Apoptosis and DNA Replication by Differential Labeling of DNA Strand breaks with Fluorochromes of Different Color Exp. Cell Res. 222:1 p.28-37

Shinozaki, R., Inoue, S., and Choi, K. (1998) Flow Cytometric Measurement of Benzo[a]pyrene-diol-epoxide-DNA Adducts in Normal Human Peripheral Lymphocytes and Cultured Human Lung Cancer Cells Cytometry 31:4 p.300-306

Holm, M., Thomsen, M., Hoyer, M., Hokland, P. (1998) Optimization of a Flow Cytometric Method for the Simultaneous Measurement of Cell Surface Antigen, DNA Content, and In Vitro BrdUrd Incorporation into Normal and Malignant Hematopoietic Cells Cytometry 32:1 p.28-36

Shackney, S.E., and Shankey, T.V. (1999) Cell Cycle Models for Molecular Biology and Molecular Oncology: Exploring New Dimensions Cytometry 35:2 p.97-116

Schmid, I., Cole, S.W., Zack, J.A., and Giorgi, J.V. (2000) Measurement of Lymphocyte Subset Proliferation by Three-Color Immunofluorescence and DNA Flow Cytometry J. Immunol. Meth. 235:1-2 p.121-131

Darzynkiewicz, Z., Robinson, J., & Crissman, H. Ed.s Methods in Cell Biology Vol. 63 3^rd Edition; 2001, Academic Press, Inc., San Diego, CA, Juan, G., Traganos, F., and Darzynkiewicz, Z. Methods to Identify Mitotic Cells by Flow Cytometry p. 343-354

Endoreduplication

Shinozaki, R., Inoue, S., and Choi, K. (1998) Flow Cytometric Measurement of Benzo[a]pyrene-diol-epoxide-DNA Adducts in Normal Human Peripheral lymphocytes and Cultured human lung Cancer Cells Cytometry 31:4 p. 300-306

Sramkoski, R.M., Wormsley, S.W., Bolton, W.E., Crumpler, D.C., and Jacobberger, J.W. (1999) Simultaneous detection of Cyclin B1, p105, and DNA Content Provides Complete Cell Cycle Phase Fraction Analysis of Cells that Endoreduplicate Cytometry 35:3 p. 274-283

Jacobberger, J.W., Sramkoski, R.M., Wormsley, S.B., and Bolton, W.E. (1999) Estimation of Kinetic Cell-Cycle-Related gene expression in G1 and G2 Phases from Immunofluorescence Flow Cytometry Data Cytometry 35:3 p. 284-289

GFP - Reporter Gene Construct

Kalejta, R.F., Shenk, T., and Beavis, A.J. (1997) Use of a Membrane-Localized Green Fluorescent Protein Allows Simultaneous Identification of Transfected Cells and Cell Cycle Analysis by Flow Cytometry Cytometry 29:4 p.286-291

Lybarger, L., Dempsey, D., Patterson, G.H., Piston, D.W., Kain, S.R., and Chervenak, R. (1998) Dual-Color Flow Cytometric Detection of Fluorescent Proteins Using Single-Laser (488nm) Excitation Cytometry 31:3 p.147-152

Naramura, M., Hu, R., and Gu, H. (1998) Mice with a Fluorescent Marker for Interleukin 2 Gene Activation Immunity 9: p.209-216

Chu, Y., Wang, R., Schmid, I., and Sakamoto, K.M. (1999) Analysis with Flow Cytometry of Green Fluorescent Protein Expression in Leukemic Cells Cytometry 36:4 p.333-339

Glucose Transport Analysis

Rauchman, M.I., Wasserman, J.C., Cohen, D.M., Perkins, D.L., Hebert, S.C. Milford, E., and Gullans, S.R. (1992) Expression of GLUT-2 cDNA in human B lymphocytes: analysis of glucose transport using flow cytometry. Biochimica et Biophysica Acta 1111:2 p.231-238

Aller, C.B., Ehmann, S., Gilman-Schs, A., and Snyder, A.K. (1997) Flow cytometric analysis of glucose transport by rat brain cells. Cytometry 27:3 p.262-268

Dimitriadis, G., Maratou, E., Boutati, E., Psarre, K., Papasteriades, C., and Raptis, S.A. (2005) Evaluation of glucose transport and its regulation by insulin in human monocytes using flow cytometry. Cytometry 64A:1 p.27-33

Quantitative Fluorescence Cytometry

Bohn, B. (1976) High-sensitivity cytofluormetric quantitation of lectin and hormone binding to surfaces of living cells. Exp. Cell Res. 14: p. 294-306

Sklar, L.A., Finney, D.A., Oades, Z., Jesaitis, A., Painter, R., and Cochrane, C. (1984) The dynamics of ligand-receptor interactions. Real-time analyses of association, dissociation, and internalization of an N-formyl peptide and it's receptors on the neutrophil. J. Biol. Chem. 259:9 p. 5661-5669

Chatelier, R. and Ashcroft, R. (1987) Calibration of flow cytometric fluorescence standards using isoparametric analysis of ligand binding. Cytometry 8:6 p. 632-636

Fay, S.P., Posner, R.G., Swann, W.N., and Sklar, L.A. (1991) Real-time analysis of the assembly of ligand, receptor, and G protein by quantitative fluorescence flow cytometry. Biochemistry 30:20 p. 5066-5075

Brotherick, ., Lennard, T.W.J., Wilkinson, S.E., Cook, S., Angus, B., and Shenton, B.K. (1994) Flow cytometric method for the measurement of epidermal growth factor receptor and comparison with the radio-ligand assay. Cytometry 16: p. 262-269

Cytometry (1998) 33:2

Single Cell Preparation from Solid Tumor

Ensley, J.F., Maciorowski, Z., Pietraszkiewicz, H., Klemic, G., KuKuruga, M., Sapareto, S., Corbett, T., and Crissman, J. (1987) Solid Tumor Preparation for Flow Cytometry Using a Standard Murine Model Cytometry 8:5 p.479-487

Ensley, J.F., Maciorowski, Z., Pietraszkiewicz, H., Hassan, M., Kish, J., Al-Sarraf, M., Jacobs, J., Weaver, A., Atkinson, D., and Crissman, J. (1987) Solid Tumor Preparation for Clinical Application of Flow Cytometry Cytometry 8:5 p.488-493

Koopman, L.A., Corver, W.E., van der Slik, A.R., Giphart, M.J., and Fleuren, G.J. (2000) Multiple Genetic Alterations Cause Frequent and Heterogeneous Human Histocompatability Leukocyte Antigen Class I Loss in Cervical Cancer J. Exp. Med. 191:6 p. 961-975

Sorting and mRNA

Diez, C., Bertsch, G., and Simm, A. (1999) Isolation of full-size mRNA from cells sorted by flow cytometry. J. Biochem. Biophys. Methods 40:3 p.69-80

Cell Viability

Perfetto, S.P., Chattopadhyay, P. K., Lamoreaux, L., Nguyen, R., Ambrozak, D., Koup, R. A., and Roederer, M. (2006) Amine reactive dyes: An effective tool to discriminate live and dead cells in polychromatice flow cytometry. J. Immunol. Meth. 313:1-2 p.199-208

Schmid, I., Ferbas, J., Uittenbogaart, C.H., and Giorgi, J.V. (1999) Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability. Cytometry 35:1 p. 64-74

Center Members Publication’s that Utilized the Services of Facility Core 4

1. Acquaah-Mensah G.K., Kehrer J.P., and Leslie S.W. (2002) In utero ethanol suppresses cerebellar activator protein-1 and nuclear factor-kappa B transcriptional activation in a rat fetal alcohol syndrome model. J. Pharmacol. Exp. Ther. 301:1 p. 277-283

2. Akhter, S., Richie, C.T., Deng, J.M., Brey, E., Zhang, X., Patrick, C. Jr., Behringer, R.R., and Legerski, R.J. (2004) Deficiency in SNM1 abrogates an early mitotic checkpoint induced by spindle stress. Molec. Cell. Biol. 24:23 p. 10448-10455

3. Atarod, E.B., and Kehrer, J.P. (2004) Dissociation of oxidant production by peroxisome proliferators-activated receptor ligands from cell death in human cell lines. Radic. Biol. Med. 37:1 p.36-47

4. Bednarek, A.K., Keck-Waggoner, C.L., Daniel, R.L., Laflin, K.J., Bergsagel, P.L., Kiguchie, K., Brenner, A.J., and Aldaz, C.M. (2001) WWOX, the FRA 16D gene, behaves as a suppressor of tumor growth. Cancer Res. 61:22 p. 8068-8073

5. Biswal S., Acquaah-Mensah G., Datta K., Wu X., and Kehrer J.P. (2002) Inhibition of cell proliferation and AP-1 activity by acrolein in human A549 lung adenocarcinoma cells due to thiol imbalance and covalent modifications. Chem. Res. Toxicol. 15:2 p.180-186

6. Bol D.K., Rowley R.B., Ho C.P., Pilz B., Dell J., Swerdel M., Kiguchi K., Muga S., Klein R., and Fischer S.M. (2002) Cyclooxygenase-2 overexpression in the skin of transgenic mice results in suppression of tumor development. Cancer Res. 62:9 p. 2516-2521

7. Chandra, D., Choy, G., Deng, X., Bhatia, B., Daniel, P., and Tang, D.G. (2004) Association of active caspase 8 with the mitochondrial membrane during apoptosis: potential roles in cleaving BAP31 and caspase 3 and mediating mitochondrion-endoplasmic reticulum cross talk in etoposide-induced cell death. Mol. Cell Biol. 24:15 p.6592-6607

8. El Kassar, N., Lucas, P.J., Klug, D.B., Zamisch, M., Merchant, M., Bare, C.V., Choudhury, B., Sharrow, S.O., Richie, E., Mackall, C.L., and Gress, R.E. (2004) A dose effect of IL-7 on thymocytye development. Blood 104:5 p.14-19-1427

9. Frankel A., Yadav N., Lee J., Branscombe T.L., Clarke S., and Bedford M.T. (2002) The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J. Biol. Chem. 277:5 p.3537-3543

10. Giammona C.J., Sawhney P., Chandrasekaran Y., and Richburg J.H. (2002) Death receptor response in rodent testis after mono-(2-ethylhexyl) phthalate exposure. Toxicol. Appl. Pharmacol . 185:2 p.119-127

11. Gomez, G., Clarkin, K.Z., Kraig, E., Infante, A.J., and Richie, E.R. (2000) TCR v(beta) repertoire restriction and lack of CDR3 conservation implicate TCR-superantigen interactions in promoting the clonal evolution of murine thymic lymphomas. Int. Immunol. 12:3 p. 263-270

12. He, G., Thuillier, P., and Fischer, S.M. (2004) Troglitazone inhibits cyclin D1 expression and cell cycling independently of PPARgamma in normal mouse skin keratinocytes. J. Invest. Dermatol. 123:6 p.1110-1119

13. Hodges L.C., Cook J.D., Lobenhofer E.K., Li L., Bennett L., Bushel P.R., Aldaz C.M., Afshari C.A., and Walker C.L. (2003) Tamoxifen functions as a molecular agonist inducing cell cycle-associated genes in breast cancer cells. Mol. Cancer Res. 1:4 p.300-311

14. Kern J.C., and Kehrer J.P. (2002) Acrolein-induced cell death: a caspase-influenced decision between apoptosis and oncosis/necrosis. Chem. Biol. Interact. 139:1 p.79-95

15. Kiguchi K., and DiGiovanni J. (2002) Animal model for gallbladder carcinoma (review). Pathology and Clinic(Japanese) 20: p.513-519

16. Kim, J., Lee, J., Yadav, N., Wu, Q., Carter, C., Richard, S., Richie, E., and Bedford, M.T. (2004) Loss of CARM1 results in hypomethylation of thymocyte cyclic AMP-regulated phosphoprotein and deregulated early T cell development. J. Biol. Chem. 279:24 p.25339-25344

17. Kleymenova, E.V., Ibraghimov-Beskrovnaya, O., Kugoh, H., Everitt, J.I., Xu, H., Kiguchi, K., Landes, G., Harris, P., and Walker, C.L. (2001) Tuberin-dependent membrane localization of polycystin-1: a functional link between polycystic kidney disease and the TSC2 tumor suppressor gene. Mol. Cell 7:4 p. 823-832

18. Klug, D.B., Carter, C., Gimenez-Conti, I.B., and Richie, E.R. (2002) Cutting edge: thymocyte-independent and thymocyte-dependent phases of epithelial patterning in the fetal thymus. J. Immunol. 169:6 p. 2842-2845

19. Klug, D.B., Crouch, E., Carter, C., Coghlin, L., Conti, C.J., and Richie, E.R. (2000) Transgenic expression of cyclin D1 in thymic epithelial precursors promotes epithelial and T cell development. J. Immunol. 164: p. 1881-1888

20. Lee J., and Bedford M.T. (2002) PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Rep. 3:3 p.268-273

21. Leonard D., Ajuh P., Lamond A.I., and Legerski R.J. (2003) hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing. Biochem. Biophys. Res. Commun. 308:4 p.793-801

22. Liu N., Kuang X., Kim H.T., Stoica G., Qiang W., Scofield V.L., and Wong P.K. (2004) Possible involvement of both endoplasmic reticulum- and mitochondria-dependent pathways in MoMuLV-ts1-induced apoptosis in astrocytes. J. Neurovirol. 10:3 p.189-198

23. Matsumoto T., Jiang J., Kiguchi K., Carbajal S., Rho O., Gimenez-Conti, I., Beltran, L., and DiGiovanni, J. (2002) Overexpression of a constitutively active form of c-src in skin epidermis increases sensitivity to tumor promotion by 12-O-tetradecanoylphorbol-13-acetate. Mol. Carcinog. 33:3 p.146-155

24. Matsumoto T., Jiang J., Kiguchi K., Ruffino L., Carbajal S., Beltran L., Bol D.K., Rosenberg M.P., and DiGiovanni J. (2003) Targeted expression of c-Src in epidermal basal cells leads to enhanced skin tumor promotion, malignant progression, and metastasis. Cancer Res. 63:16 p.4819-4828

25. Poetschke, H.L., Klug, D.B., Perkins, S.N., Wang, T.T., Richie, E.R., and Hursting, S.D. (2000) Effects of calorie restriction on thymocyte growth, death and maturation. Carcinogenesis 21:11 p. 1959-1964

26. Powers J.T., Hong S., Mayhew C.N., Rogers P.M., Knudsen E.S., and Johnson D.G. (2004) E2F1 uses the ATM signaling pathway to induce p53 and Chk2 phosphorylation and apoptosis. Mol Cancer Res . 2:4 p.203-214

27. Qiang, W., Cahill, J.M., Liu, J., Kuang, X., Liu, N., Scofield, V.L., Voorhees, J.R., Reid, A. J., Yan, M., Lynn, W.S., And Wong, P.K.Y. (2004) Activation of Transcription Factor Nrf-2 and Its Downstream Targets in Response to Moloney Murine Leukemia Virus ts1-Induced Thiol Depletion and Oxidative Stress in Astrocytes J. Virol. 78:21 p.11926-11938

28. Richburg J.H., Johnson K.J., Schoenfeld H.A., Meistrich M..L, and Dix D.J. (2002) Defining the cellular and molecular mechanisms of toxicant action in the testis. Toxicol. Lett . 135:3 p.167-183

29. Richie, E.R., Schumacher, A., Angel, J.M., Hollowya, M., Rinchik, E.M. and Magnuson, T. (2002) The polycomb-group gene eed regulates thymocyte differentiation and suppresses the development of crciongen-induced T-cell lymphomas. Oncogene 21:2 p.299-306

30. Sano, S., Chan, K.S., Carabajal, S., Clifford, J., Peavey, M., Kiguchi, K., Itami, S., Nickoloff, B.J., and DiGiovanni, J. (2005) Stat3 links activated keratinocytes and immunocytes required for development of psoriasis in a novel transgenic mouse model. Nature Med. 11:1 p.43-49

31. Tang D.G., La E., Kern J., and Kehrer J.P. (2002) Fatty acid oxidation and signaling in apoptosis. Biol. Chem. 383:(3-4) p.425-442

32. Thuillier P., Brash A.R., Kehrer J.P., Stimmel J.B., Leesnitzer L.M., Yang P., Newman R.A., and Fischer S.M. (2002) Inhibition of peroxisome proliferator-activated receptor (PPAR)-mediated keratinocyte differentiation by lipoxygenase inhibitors. Biochem J . 366:(Pt 3) p.901-910

33. Tong, Z., Wu, X., and Kehrer, J.P. (2003) Increased expression of the lipocalin 24p3 as an apoptotic mechanism for MK886. J. Biochem. 372 (part 1) p. 203-210

34. Wang, A., Gu, J., Judson-Kremer, K., Powell, K.L., Mistry, H., Simhambhatia, p., Aldaz, C.M., Gaddis, S., and MacLeod, M.C. (2003) Response of human mammary epithelial cells to DNA damage induced by BPDE: involvement of novel regulatory pathways. Carcinogenesis 24:2 p. 225-234

35. Wu, X., Biswal, S.S., and Kehrer, J.P. (2003) Roles of 5-lipoxygenase activating protein in cell proliferation and apoptosis. Cell Biol. Toxicol. 19:3 p. 135-143

36. Yan, M., Kuang, X., Qiang, W., Shen, J., Claypool, K., Lynn, W.S., and Wong, P.K. (2002) Prevention of Thymic Lymphoma Development in Atm-/- Mice by Dexamethasone Cancer Res. 62:18 p.5153-5157

37. Yu W., Sanders B.G., and Kline K. (2002) RRR-alpha-tocopheryl succinate induction of DNA synthesis arrest of human MDA-MB-435 cells involves TGF-beta-independent activation of p21Waf1/Cip1. Nutr. Cancer 43:2 p.227-236

38. Yu W., Sanders B.G., and Kline K. (2003) RRR-alpha-tocopheryl succinate-induced apoptosis of human breast cancer sells involves Bax translocation to mitochondria. Cancer res. 63:10 p. 2483-2491

39. Zhang, S., Succi, J., Feng, Z., Prithivirajsingh, S., Story, M.D., and Legerski, R.J. (2004) Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damagae checkpoint response. Molec. Cell. Biol. 24:20 p. 9207-9220

40. Zamisch, M., Moore-Scott, B., Su, D., Lucas, P.J.,Manley, N., and Richie, E. (2005) Ontogeny and regulation of IL-7 expressing thymic epithelial cells. J. Immunol. 174:1 p.60-67

41. Patrawala, L., Calhoun, T., Schneider-Broussard, R., Zhou, J., Claypool, K., and Tang, D. (2005) Side Population is Enriched in Tumorigenic, Stem-Like Cancer Cells, whereas ABCG2+ and ABCG2- Cancer Cells are Similarly Tunmorigenic. Cancer Res. 65:14 p. 1-13

42. Yan, M., Zhu, C., Liu, N., Jiang, Y., Scofield, V.L., Riggs, P.K., Qiang, W., Lynn, W.S., and Wong, P.K.Y. (2006) ATM controls c-Myc and DNA synthesis during postnatal thymocyte development through regulation of redox state. Free Radical Biology & Medicine 41:4 p. 640-680

43. Li, H., Chen, X., Calhoun-Davis, T., Claypool, K., and Tang, D.G. (2008) PC3 Human Prostate Carcinoma Cell Holoclones Contain Self-renewing Tumor-Initiating Cells. Cancer Res. 68:6 p. 1820-1825

Cytometry Software

Coulter^® Expo^TM32 Software Program VER 4.02: FCS listmode (DOS) format

Coulter^® EPICS^® Elite Software Program VER 4.02: FCS listmode (DOS) format

Verity Software House, Inc. WinList^TM Software Program VER 5.0

FlowJo V6.2/5.5, Tree Star, Inc.

BD Biosciences Diva 6.0

DNA Analysis Software

Rabinovitch, Peter, S., Ph.D.: MultiCycle 1994 University of Washington, Seattle, WA Distributed by Phoenix Flow Systems, Inc.

Verity Software House, Inc. ModFit^TM Software Program VER 3.0

Image Capture Software

Pizazz Plus, Application Techniques, Inc. Pepperell, MA

Graphics Software

Adobe^® PhotoShop 5.5 Macromedia^® Free Hand 9.0 Microsoft^® PowerPoint

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Last updated: 15-May-08