Histochemistry
Background: tissue structures
and substances are colored when a chemical group (e.g. carboxyl, phosphoric
or aldehyde) reacts with the stain (see examples
below).
Suitable for : diagnosis
of pathologic conditions.
Not suitable for : routine
tissue staining.
Masson’s trichrome staining of formalin-fixed mouse
lung: cytoplasm and muscle fibers are red, collagen and mucin are blue,
and nuclei are black.
Staining of small intestine (mouse, formalin-fixed)
with mucicarmine solution turns mucin deep rose to red, nuclei black
and other tissue elements yellow.
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