T Cell Subsets and DNA Content on Normal Mouse Thymus

Dean Tang, Ph.D.; Research Core 5: Mechanisms of Disease Prevention

Paul Wong, Ph.D.& Mingshan Yan, M.D. Research Core 1: Mechanisms of Toxicity and Cell Death

DNA Content (7-AAD)

MultiCycle, Phoenix Flow Systems, Inc., San Diego, CA

2-Color Cell Surface Immunophenotyping & DNA Content Protocol

References

Schmid, I., Uittenbogaart, C.H., and Giorgi, J.V. (1991) A Gentle Fixation and Permeabilization Method for Combined Cell Surface and Intracellular Staining With Improved Precision in DNA Quantification Cytometry 12: pp. 279-285

Rabinovitch, P.S., Torres, R.M., and Engel, D. (1986) Simultaneous Cell Cycle Analysis and Two-Color Surface Immunofluorescence Using 7-Amino-Actinomycin D and Single Laser Excitation: Applications to Study of Cell Activation and the Cell Cycle of Murine Ly-1 B Cells. J. Immunol. 136:8 pp.2769-2775

Cell Surface Labeling Aggregates

3-Color Intracellular BrdU and Cell Surface DNA Content 2-Color Cell Cycle Bacteria

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Last updated: 11-March-02

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