Animal Resources
Significance and Description
Animal models are extremely important tools for studying the molecular mechanisms that underlie complex biological processes. Research using animal models has provided important components of our understanding of how cancer arises and has led to the development of new ways to detect, treat, and prevent cancer. Transgenic and gene-targeting technologies can now be used to quickly and efficiently generate animals that mimic certain aspects of human disease. Genetically defined mouse models are also proving to be very useful for studying how specific genetic alterations or naturally occurring polymorphisms interact with the environment to induce disease. Three animal resource components at Science Park - Research Division provide comprehensive support for research projects utilizing rodent and aquatic animal models: Research Animal Support Facility, Genetics Services and Transgenic Services.
Research Animal Support Facility
The Department's Animal Resources are housed in the Research Animal Support Facility - Smithville (RASF-S) that is supported in part by the Institution's Cancer Center Support Grant from the National Cancer Institute. This facility is one of about 700 worldwide that is accredited by AAALAC International (Association for Assessment and Accreditation of Laboratory Animal Care International). AAALAC International is a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. Our participation in this voluntary program is a demonstration of our sincere commitment to responsible animal care and use. In addition, we comply with all guidelines and requirements set forth by the Public Health Service (PHS) Policy on Humane Care and Use of Laboratory Animals, The U.S. Department of Health and Human Services Guide for the Care and Use of Laboratory Animals, and as applicable, the USDA Animal Welfare Act. Our institution has an Assurance Statement on file with the PHS Office of Laboratory Animal Welfare (OLAW) that describes institutional policy and procedure in adhering to these laws and standards.
The Research Animal Support Facility provides animal husbandry services, veterinary care and consultation, surgical and technical support, and numerous genetic and diagnostic services. Regulatory compliance and training and education in animal care and use are additional functions of the program, as are provision of animal health quality assurance testing, breeding colony management, genetic consultation, genetic authentication, and application of international standards of nomenclature for animal models.
Genetic Services
Genetic Services provides UTMDACC investigators with cost-effective, customized genome scans using polymorphic genetic markers, to support speed congenic development, background strain characterization, genetic quality control, and genetic linkage analysis for mice and rats. Consulation on rodent genetics is also provided by this component.
Many new lines of mutant and genetically modified mice and rats, whether developed at UTMDACC or obtained from outside the Institution, are initially on a mixed genetic background. It is well known that the genetic background influences the phenotype of transgenic and knock out (KO) models (e.g., the well-documented case of epidermal growth factor receptor null mice). We anticipate that there will be an increase in studies to identify and characterize genes that modify these phenotypes. In addition, in many cases investigators need to create congenic lines to study the effects of a particular genetic allele in a different strain. Genome scans using multiple, informative polymorphic loci represent the state-of-the-art in defining genetic background.
Mutant Mouse Pathology Services
The Mutant Mouse Pathology Service provides UTMDACC investigators with cost-effective anatomical pathology assistance, including gross necropsy and histopathology performed by experienced veterinary pathologists. Consultation on animal models selection and validation is also provided.
New lines of mutant and genetically modified mice are being developed and employed in ever increasing numbers for biomedical research. However, careful characterization of mutant mice, their validation as models of specific human diseases, and the appropriate application of these models require expert knowledge of mouse biology and comparative pathology. The Mutant Mouse Pathology Service, which is staffed by three experienced veterinary pathologists, can provide this expertise. The Service also offers consistent, reproducible semi-quantitative histopathologic evaluation of samples from large experimental studies.
Specific services provided are:
- Ante mortem and post mortem imaging
- Hematology and serum chemistry
- Necropsy
- Histopathology
- Consultation
- Training
Transgenic Services
This component provides investigators with a cost-effective means of developing genetically engineered animal models.
Specific services provided are:- Generation of transgenic mouse founders by pronuclear microinjection of DNA constructs into fertilized eggs in either the FVB or SKH1 strain background
- Generation of chimeric mice by blastocyst injection of gene-targeted ES cell lines
- Rederivation of transgenic and other specialized mouse strains by embryo transfer
- Archival storage of frozen embryos and sperm from transgenic and other specialized mouse strains
- Consultation, technical support and training to Investigators wishing to establish their own genetically modified animals
Staff
Research Animal Support Facility
Lezlee Coghlan D.V.M., Diplomate, Am. College of Laboratory Animal Medicine
Associate Professor
Director, Research Animal Support Facility
Dale Weiss, Certified Laboratory Animal Technologist
Operations Manager, Research Animal Support Facility
Genetic Services
Fernando Benavides D.V.M., Ph.D., Diplomate, Am. College of Laboratory Animal Medicine
Associate Professor
Director, Genetic Services
Joe M. Angel, Ph.D.
Associate Professor
Co-Director, Genetic Services
Mutant Mouse Pathology Services
Donna Kusewitt, D.V.M., Ph.D., A.C.V.P.
Professor
Director, Mutant Mouse Pathology Service
Mark McArthur, D.V.M., A.C.V.P.
Associate Professor
Co-Director, Mutant Mouse Pathology
Claudio Conti, D.V.M., Ph.D.
Professor
Carlos Perez., D.V.M.
Research Investigator
Mutant Mouse Pathology Service
R&D Coordinator, RASF Genetic Services
Transgenic Services
David G. Johnson, Ph.D.
Professor
Director, Transgenic Services